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LEE, SHAO-CHEN
Associate Professor
073798@mail.fju.edu.tw
Education
Ph.D., Department of Life-Sciences, National TsingHua University, Hsinchu, Taiwan

Experience
2015.10~present     Coordinator for the International program Affairs, College of Medicine, FuJen Catholic University
2015.08~present     Associate Professor, School of Medicine, College of Medicine, FuJen Catholic University
2008.02~2015.07     Assistant Professor, School of Medicine, College of Medicine, FuJen Catholic University
2007.10~2008.02     Postdoctoral fellow in Institute of Bioinformatics and Structural Biology, National TsingHua University, Taiwan.
2006.02-2007.08     Postdoctoral fellow in Sunnybrook health science center, Toronto, ON., CANADA.
2004.08-2006.02     Postdoctoral fellow in Institute of Bioinformatics and Structural Biology, National TsingHua University, Taiwan.
2002.06-2002.09     Exchange summer student at Institute for Biological-Sciences, Ottawa, National Research Council for NSC-NRC Joint Project between Taiwan and CANADA.
2001.06-2001.09     Exchange summer student at Institute for Biological-Sciences, Ottawa, National Research Council for NSC-NRC Joint Project between Taiwan and CANADA.
2000.06-2000.08     Exchange summer student at Institute for Biological-Sciences, Ottawa, National Research Council for NSC-NRC Joint Project between Taiwan and CANADA.
1998.02-1998.06     Teaching assistant in Biochemistry Experiment, Department of Life-Sciences, National TsingHua University, Hsinchu, Taiwan

Specialty
Biochemistry, Glycobiology, Molecular recognition, Tumor Biology

　Author
Journal Paper
1. Shih, C.Y., Cheng, Y.C., Hsieh, C.H., Tseng, T.T., Jiang, S.S., and Lee, S.C. (2020) Drug-selected population in melanoma A2058 cells as melanoma stem-like cells retained angiogenic features – the potential roles of heparan-sulfate binding ANGPTL4 protein. Aging, accepted
2. Cheng, Y.C., Ku,W.C., Tseng, T.T., Wu, C.P., Li, M., and Lee, S.C. (2020) Anchorage independence altered vasculogenic phenotype of melanoma cells through downregulation in aminopeptidase N /syndecan-1/integrin β4 axis. Aging, 12, 16803-16819.
3. Lin, S.C., Wu, C.P., Tseng, T.T., Jhang, Y., and Lee, S.C. (2020) Role of syndecan-1 and exogenous heparin in hepatoma sphere formation. Biochemistry and cell biology, 98, 112-119. 
4. Uen, Y.H., Wang, J.W., Wang, C.C., Jhang, Y., Chung, J.Y., Tseng, T.T., Sheu, M.J, and Lee, S.C. (2018) Mining of potential microRNAs with clinical correlation - regulation of syndecan-1 expression by miR-122-5p altered mobility of breast cancer cells and possible correlation with liver injury. Oncotarget, 9, 28165-28175.
5. Tseng, T.T., Uen, W.C., Tseng, J.C., and Lee S.C. (2017) Enhanced chemosensitization of anoikis-resistant melanoma cells through syndecan-2 upregulation upon anchorage independency. Oncotarget, 8, 61528-61537. 
6. Uen, W.C., Hsieh, C.H., Tseng, T.T., Jiang, S.S., Tseng, J.C., and Lee, S.C. (2015) Anchorage-independency promoted tumor malignancy of melanoma cells under reattachment through elevated interleukin-8 and CXC chemokine receptor 1 expression. Melanoma Res. 25, 35-46.
7. Wang,C.C., Tseng,T.T., Jhang,Y., Tseng,J.C., Hsieh,C.H., Wu,W.G., and Lee, S.C. (2014) Loss of cell invasiveness through PKC-mediated syndecan-1 downregulation in melanoma cells under anchorage-independency. Exp. Dermatol. 23, 843-849. 
8. Leu, F.J., Huang, C.L., Sue, Y.M., Lee, S.C., and Wang, C.C. (2014) Effects of tattoo ink’s absorption spectra and particle size on cosmetic tattoo treatment efficacy using Q-switched Nd:YAG laser. Laser Med. Sci. (accepted).
9. Lee, S.C., Lin, C.C., Wang, C.H., Wu, P.L., Huang, H.W., Chang, C.I., and Wu, W.G. (2014) Endocytotic routes of cobra cardiotoxins depend on spatial distribution of positively charged and hydrophobic domains to target distinct types of sulfated glycoconjugates on cell surface. J. Biol. Chem. 289, 20170-20181.
10. Wang, C.C., Huang, C.L., Lee, S.C., Sue, Y.M., and Leu, F.J. (2013) Treatment of cosmetic tattoos with nonablative fractional laser in an animal model: a novel method with histopathologic evidence. Lasers in Surgery and Medicine 45, 116-122.
11. Wang, C.C., Huang, C.L., Sue, Y.M., Lee, S.C., and Leu, F.J. (2012) Treatment of cosmetic tattoos using carbon dioxide ablative fractional resurfacing in an animal model: A novel method confirmed histopathologically. Dermatologic Surgery 2013, 39,571-577.
12. Chen, F.F., Lin, W.H., Lin, S.C., Kuo, J.H., Chu, H.Y., Huang, W.C., Chuang, Y.J., Lee, S.C., and Sue SC. (2012) Significance of heparin binding to basic residues in homologous to the amino terminus of hepatoma-derived growth factor and related proteins. Glycobiology 22, 649-661.
13. Lee, S.C., Fang, L., Wang, C.H., Kahai, S., Deng, Z., and Yang, B.B. (2011) A non-coding transcript of nephronectin promotes osteoblast differentiation by modulating microRNA functions. FEBS Lett. 585, 2610.
14. Wang, C.H., Davamani, F., Sue, S.C., Lee, S.C., Wu, P.L., Tang, F.M., Shih, C., Huang, T.H., and Wu, W.G. (2011) Cell surface heparan sulfates mediated internalization of PWWP/HATH domain of HDGF via macropinocytosis to fine-tune cell signalling processes involved in fibroblast cell migration. Biochem J. 433, 127.
15. Wang, Y.L., Kuo, J.H., Lee, S.C., Liu, J.S., Hsieh, Y.C., Shih, Y.T., Chen, C.J., Chiu, J.J., and Wu, W.G. (2010) Cobra CRISP functions as an inflammatory modulator via a novel Zn2+- and heparan sulfate- dependent transcriptional regulation of endothelial cell adhesion molecules. J. Biol. Chem. 285, 37872-37883.
16. Chen, C.P., Hung, C.F., Lee, S.C., Lo, H.M., Wu, P.H., Wu, W.B. (2010) Lycopene Binding Compromised PDGF-AA/-AB Signaling and Migration in Smooth Muscle Cells and Fibroblasts: Prediction of The Possible Lycopene Binding Site within PDGF. Naunyn-Schmiedeberg Archives of Pharmacol 381, 401-414.
17. Wang, C.C., Huang, C.L., Yang, A.H., Chen, C.K., Lee, S.C., and Leu, F.J. (2010) Comparison of two Q-switched lasers and a short-pulsed erbium-doped yttrium aluminum garnet laser for treatment of cosmetic tattoos containing titanium and iron in an animal model. Dermato. Surg. 36, 1656-1663.
18. Kahai, S., Lee, S.C., Seth, A., and Yang, B.B. (2010) Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats. FEBS Lett. 584, 233.
19. Kahai, S.*, Lee, S.C. *, Lee, D.Y. *, Yang, J., Li, M., Wang, C.H., Zhang, Y., Peng, C., and Yang, B.B. (2009) MicroRNA miR-378 regulates nephronectin expression modulating osteoblast differentiation by targeting GalNT-7. PloS One. 4, e7535 (*: equal contribution).
20. Wang, C.H., Lee, D.Y., Deng, Z., Jeyapalan, Z., Lee, S.C., Kahai, S., Lu, W.Y., Zhang, Y., and Yang, B.B. (2008) MicroRNA miR-328 regulates zonation morphogenesis by targeting CD44 expression. PLoS One, 3, e2420.
21. Sue, S.C., Lee, W.T., Tien, S.C., Lee, S.C., Yu, J.G., Wu, W.J., Wu, W.G., and  Huang, T.H. (2007) PWWP Module of Human Hepatoma-derived Growth Factor Forms a Domain-swapped Dimer with Much Higher Affinity for Heparin. J. Mol. Biol. 367,456-472.
22. Wu, P.L., Lee, S.C., Chuang, C.C., Mori, S., Akakura, N., Wu, W., and Takada, Y. (2006) Non-cytotoxic cobra cardiotoxin A5 binds to integrin alpha vbeta 3 and inhibits bone resorption: Identification of cardiotoxins as non-RGD integrin- binding proteins of the Ly-6 family. J. Biol. Chem. 281, 7937-7945.
23. Lee, S.C., Chien, L.F., Van, R.C., Hsiao, Y.Y., Hong, J.L., and Pan, R.L. (2006) Radiation inactivation analysis of thylakoid protein kinase systems in light and in darkness. Photosynthetica 44, 116-124.
24. Wang, C.H., Liu, J.H., Lee, S.C., Hsiao, C.D., and Wu, W. (2006) Glycosphingolipid-facilitated membrane insertion and internalization of cobra cardiotoxin. The sulfatide.cardiotoxin complex structure in a membrane-like environment suggests a lipid-dependent cell-penetrating mechanism for membrane binding polypeptides. J. Biol. Chem. 281, 656-667. 
25. Wang, C.H., Monette, R., Lee, S.C., Morley, P. and Wu, W. (2005) Cobra cardiotoxin-induced cell death in fetal rat cardiomyocytes and cortical neurons: different pathway but similar cell surface target. Toxicon 46, 430-440.
26. Lee, S.C., Guan, H.H., Wang, C.H., Huang, W.N., Tjong, S.C., Chen, C.J., and Wu, W. (2005) Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3. J. Biol. Chem. 280, 9567-9577.
27. Sue, S.C., Chen, J.Y., Lee, S.C., Wu, W., and Huang, T.H. (2004) Solution structure and heparin interaction of human hepatoma-derived growth factor. J. Mol. Biol. 343, 1365-1377.
28. Sue, S.C., Brisson, J.R., Chang, S.C., Huang, W.N., Lee, S.C., Jarrell, H.C., and Wu, W. (2001) Structures of heparin-derived disaccharide bound to cobra cardiotoxins: context-dependent conformational change of heparin upon binding to the rigid core of the three-fingered toxin. Biochemistry 40, 10436-10446.
29. Lin, Y.H., Huang, W.N., Lee, S.C., and Wu, W. (2000) Heparin reduces the alpha-helical content of cobra basic phospholipase A2 and promotes its complex formation. Int. J. Biol. Macromol. 27, 171-176.
30. Lin, Y.H., Lee, S.C., Chang, P.Y., Rajan, P.K., Sue, S.C., and Wu, W. (1999) Heparin binding to cobra basic phospholipase A2 depends on heparin chain length and amino acid specificity. FEBS Lett. 453, 395-399.

Conference Paper
1. Shih, C.Y., Liu,J.Y., Tseng, T.T., and Lee, S.C. (2018) The roles and regulation of Angiopoietin-like 4 protein in drug-resistant melanoma stem-like cells. BC050, the 33th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
2. Wu, C.P., and Lee, S.C. (2018) The alterations of extracellular matrix components in melanoma cells under hypoxia and anchorage independency. BC057, the 33th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
3. Li, C.Y., Tseng, T.T., Wu, C.P., Chung, J.Y., and Lee, S.C. (2018) Screening and validation of XAF1 as potential target regulated by exosomal miRNA-1976. BC059, the 33th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
4. Hsieh, C.H., Tseng, T.T., and Lee, S.C. (2018) Exploring the role of Syndecan-2 overexpression in chemosensitivity of melanoma cells toward proteasome inhibition. BC079, the 33th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
5. Chou, K.T, Tseng, T.T., Wang, J.W., Uen, Y.H., and Lee, S.C. (2015) Exosomal microRNAs modulating cellular PI3K-AKT pathway act as biomarker of liver cell injury. P081, the 30th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
6. Tseng, T.T, Jhang, Y., Tseng, J.C., and Lee, S.C. (2015) Anchorage-independency alters vascular mimicry phenotypes of melanoma tumors through SDC1/integrin beta4 downregulation. P536, the 30th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
7. Tseng, T.T, Jhang,Y., Tseng, J.C., and Lee, S.C. (2014) Roles of syndecan-2 upregulation in anoikis-resistant human melanoma cells. P820, The 29th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
8. Hsieh, C.H., Tseng, T.T, Hsu, L.L, and Lee, S.C. (2014) Characterization of the drug-resistant population in melanoma A2058 cells as melanoma stem-like cells. P137, The 29th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
9. Hsieh, C.H., Tseng, T.T., Tseng, J.C., and Lee, S.C. (2013) Anchorage-independent growth of melanoma cells affected malignancy by shift of IL-8/IL-8Rs functional axis. P961, The 28th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
10. Tseng, T.T., Tseng,J.C., Hsieh, C.H., Jhang, Y., and Lee, S.C. (2013) Anchorage- independent growth of melanoma cells contributes to loss of invasiveness through syndecan-1 downregulation. P715, The 28th Joint Annual Conference of Biomedical Science. Taipei, Taiw
11. Jhang, Y., Tseng, J.C., and Lee, S.C. (2013) Strategy and analysis of microRNA regulation on syndecan-1 expression in breast cancer cells. P683, The 28th Joint Annual Conference of Biomedical Science. Taipei, Taiwan.
12. Lee, S.C., Wang, C.H., and Wu, W. (2005) Distinct effect of low and high sulfated heparan sulfate domain in mediating cell internalization of cobra cardiotoxin homologues. Meeting in “Proteoglycan in cell signaling”, Stockholm, Sweden.
13. Lee, S.C., Guan, H.H., Wang, C.H., Huang, W.N., Tjong, S.C., Chen, C.J., and Wu, W. (2004) Structural basis of citrate-dependent heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3. 155, US/Japan Glyco 2004. Honolulu, HI, USA.
14. Sue, S.C., Li, W.T., Lin, Y.C., Yu, J.G., Lee, S.C., Wu, W., Chen, J.Y., and Huang, T.H. Heparin-mediated cellular internalization of hepatoma-derived growth factor: role of the conserved N-terminal HATH domain. 194, US/Japan Glyco 2004. Honolulu, HI, USA.
15. Wang, C-H., Wu, P-L., Huang, W-N., Lee, S.C., Monette, R., Morley, P., Tanphaichitr, N., and Wu, W. (2004) Sulfatide promoted oligomerization of cobra cardiotoxin in lipid raft domains responsive for its cell effects. The 6th Congress of International Society for
16. Sue, S-C., Li, W-T., Lin, Y-C., Lee, S.C., Wu, W., Chen, J-Y., and Huang, T-H. (2004) Structural and functional relationship of HATH domain of Human Hepatoma-derived Growth Factor. PS20, The 9th Symposium on Recent Advances in Biophysics. Taipei, Taiwan, R.O.C.
17. Guan, H-S., Lee, S-C., Wang, C-H., Huang, W-N., Wu, W., and Chen, C-J. (2004) Crystal Structure of CTX A3 and Hexasaccharide Heparin Complex from Naja Atra. PS23, The 9th Symposium on Recent Advances in Biophysics. Taipei, Taiwan, R.O.C.
18. Guan, H-S., Lee, S-C., Wu, W., and Chen, C-J. (2003) Crystal structure of CTX A3 and hexasaccharide heparin complex from Naja atra. PS17, The 4th East Asian Biophysics Symposium. Taipei, Taiwan, R.O.C.
19. Wang, Y-L., Lee, S-C., Goh, K-X., Wu, W., and Chen, C-J. (2003) Purification and Crystallization of Natrin, a Cystein-rich Secretory Protein, from Naja atra. PS18, The 4th East Asian Biophysics Symposium. Taipei, Taiwan, R.O.C.
20. Lee, S-C., Hirama, T., MacKenzie, C.R., and Wu, W. (2003) Cobra cardiotoxin binding to heparin: specificity, cooperativity, and implications for the role of glycosaminoglycans in targeting toxin isoforms to cell surfaces. LB198, Experimental Biology Annual Meetin
21. Lee, S-C., Liao, C-H., and Wu, W. (2000) The interaction between cardiotoxins and differential heparin fragments. C-194, The 20th International Carbohydrate Symposium. Hamburg, Germany.